dNTP Mix, 40 mM

Ready-to-use molecular grade dNTP Mix containing dATP, dCTP, dGTP and dTTP

Product Description

Highly quality dNTPs are a vital requirement for successful PCR, as the presence of contaminating impurities will result in a decrease in amplification sensitivity and product yield. Bioline ultra-pure dNTPs undergo highly stringent purification steps to give a greater than 99% purity and are tested or the absence of DNase, RNase, Protease, Nickase activity. They are then quality controlled& in a variety of applications, ensuring that they are highly suitable for the most sensitive of PCR techniques.

All Bioline dNTP Mixes, designed to save hands-on time for researchers and minimize the possibility of contamination, are supplied as an equimolar solution of ultra-pure dATP, dCTP, dGTP and dTTP as lithium salts (100 mM dNTP Mix: 25 mM each, 40 mM dNTP Mix: 10 mM each, 10 mM dNTP Mix: 2.5 mM each).

Lithium salts have greater resistance to repeated freezing and thawing cycles than sodium salts and remain sterile due to the bacteriostatic activity of lithium towards various microorganisms, giving greater reliability and an extended shelf life.

• High quality - >99% purity determined by HPLC, ideal for use in PCR reactions
• Long shelf-life - no need for aliquoting
• Convenient formats - available in various concentrations, offering ease of use
• Enzyme free - DNase, RNase and Nickase free

Applications

• DNA amplification (standard, long-range, multiplex and high-fidelity PCR)
• RT-PCR
• qPCR
• Genotyping
• cDNA synthesis
• DNA sequencing
• DNA labeling

High quality deoxyribonucleotide triphosphate (dNTP) mixes in ready-to-use concentrations

Two different batches of dNTPs were freeze-thawed 5, 10 and 20 times and two-fold serial dilution was used in a PCR reaction. Both batches produced identical PCR products of 2 kb whether freeze-thawed 5 times or freeze-thawed 20 times.