100 bp HyperLadder

Ready-to-use molecular weight marker, designed for easy size determination of nucleic acids from 100 bp to 1 kb.

Product Description

HyperLadder™ 100bp is a molecular weight marker, composed of a restriction digest plus one or more PCR products, especially designed for easy size determination of linear double-stranded DNA fragments on 1% to 2% agarose gels.

The 10 regularly spaced bands, ranging from 100 bp to 1013 bp, with a doublet band at 300/311 bp and higher intensity reference bands at 1013 bp allow for easy identification and orientation of your band at a glance.

This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 100bp from the vial to the gel. The concentration of DNA in each of the HyperLadder 100bp bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The HyperLadder 100bp is also supplied with a 5x loading dye for loading of sample DNA.

HyperLadder 100bp is perfect for size determination in techniques such as confirmation of plasmid construction and PCR products as well as other downstream techniques.

• Broad size range – 10 evenly distributed bands from 100 bp to 1 kb
• Easy to orientate – several brighter reference bands
• Stable – can be stored at room temperature for up to 6 months
• Supplied with loading dye – for loading sample DNA

Ready-to-use molecular weight marker for easy size determination
5 uL HyperLadder 100bp run on a 2% TBE-agarose gel and visualized using ethidium bromide

Applications

• Restriction fragment mapping
• Determination of PCR amplicon size
• Confirmation of plasmid construction
• Detection of DNA polymorphisms
• Southern/Northern blotting
• Cloning