Uracil DNA-Glycosilase (UDGase)

Release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA 

Product Description

Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases).

• Removes uracils from DNA - causes fragmentation of DNA and stops amplification in a PCR
• Easy-to-use - novel, sensitive mutant is irreversibly inactive after heating

UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200 mM).

Bioline UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases. It originates from recombinant E. coli strain carrying the over expressed gene of Uracil DNA Glycosylase from E. coli.

Applications

• Control of carry-over contamination in PCR
• As a probe for protein-DNA interaction studies
• Cloning of PCR products