MyFi Mix, 2x

Unique blend of highly-efficient MyTaq HS DNA Polymerase and a proprietary proofreading enzyme that combine to give increased target affinity for use with challenging templates and inhibitor-rich samples.

• Robust - enzyme blend and buffering system promotes reliable amplification of the most challenging and complex targets, even in the presence of inhibitors
• Sensitive - improved target affinity and high processivity ensure successful amplification in low-copy number assays
• Efficient - high-yield amplification of a broad range of targets up to 10 kb including complex DNA extracted from human, animal and plant samples
• Specific - an antibody-mediated hot-start blend that remains completely inactive during PCR set-up to prevent non-specific amplification
• Convenient - an all-in-one-tube mastermix that improves the speed, convenience and accuracy of PCR set-up
• Accurate - proofreading component delivers 3.5x higher fidelity than Taq DNA Polymerase, enabling cloning of PCR products

Product Description

MyFi™ has been developed to give reliable amplification of targets up to 10 kb from challenging and complex targets. MyFi shows improved tolerance to PCR inhibitors, thereby enabling reliable detection from samples from which DNA is difficult to purify. Furthermore a unique buffer system and enzyme blend promote highly sensitive amplification, ideal for low-copy number targets. The proofreading ability of MyFi allows all PCR products to be cloned. The inclusion of MyTaq HS means MyFi Mix generates PCR products with 3’-A overhangs, which is perfect for TA cloning. MyFi Mix has the added convenience of room temperature reaction assembly, to avoid non-specific amplification and primer-dimer formation.

MyFi Mix is supplied as a mastermix that requires the addition of only template, primers and water, thereby reducing the number of pipetting steps during PCR set-up, for improved speed, throughput and assay reproducibility. The inclusion of dNTPs, MgCl₂ and enhancers at optimal concentrations, helps eliminate the need for optimization, thereby saving on time, cost and making MyFi Mix well suited to automation.

Applications

• Amplification of challenging and complex templates
• Inhibitor-tolerant PCR
• Longer PCR (up to 10 kb)
• Robust PCR
• Low-copy PCR
• Higher-fidelity PCR (NGS library amplification)
• TA cloning

High-yield PCR from GC-rich and complex templates

A 525 bp fragment of the 62% GC-rich human epidermal growth factor receptor (EGFR) gene (A), a 750 bp fragment of the 64% GC-rich translation factor p64 (myc) gene (B), a 900 bp fragment of 43% GC-rich angiotensin II receptor type I (AGTR1) gene (C), a 1.2 kb fragment of 62% GC-rich EGFR gene (D) were amplified using MyFi DNA Polymerase and similar polymerases from other suppliers. A five-fold serial dilution of human genomic DNA (25 ng - 8 pg, 1.6 pg and 0 pg, Lanes 1-8 respectively) was used as template. Marker is HyperLadder 1kb (M). The results demonstrate that that MyFi delivers more reliable amplification of challenging targets.