BIOTAQ DNA Polymerase

BIOTAQ is a highly purified, thermostable DNA polymerase offering good performance over a wide range of PCR templates. It is a good choice for routine PCR assays and delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.

• Good standard Taq polymerase - ideal for setting up new procedures
• Easy to use - designed for easy optimization of PCR applications
• Suitable for TA cloning - leaves 'A' overhang

Product Description

BIOTAQ™ is a purified thermostable DNA polymerase offering high yield over a wide range of PCR templates, and is a good choice for routine assays. BIOTAQ is a robust preparation and delivers high yields with minimal background. BIOTAQ possesses 5´-3´ exonuclease activity and leaves an ´A´ overhang such that the PCR product is suitable for effective integration into TA cloning vectors.

BIOTAQ is supplied with 10x NH4-based Reaction Buffer, which provides optimal conditions for most experiments. Additional MgCl₂ is provided to allow reaction conditions to be adjusted to suit the template.

Applications

• Routine PCR applications
• TA cloning

Amplification of a variety of fragments

Four different genes were amplified from mouse genomic DNA using BIOTAQ DNA Polymerase: 1.4 kb and 1.6 kb fragment of rn18s gene (lanes 1 and 2), 500 bp fragment of Fabpi gene (lane 3), 350 bp fragment of IL-2 gene (lane 4). HyperLadder 50bp (M).