Recombinant Ebolavirus (subtype Bundibugyo,strain Uganda 2007) Envelope Glycoprotein 1 (GP1)
| Specifications | |
|---|---|
| Category: | Viral Proteins |
| Molecular Weight: | 31.8 kDa |
| Tag: | C-10×His |
| Label / Conjugate: | N/A |
| Source: | Ebolavirus (subtype Bundibugyo, strain Uganda 2007) GP1 Protein, His Tag (BEP-V5221) is expressed from human 293 cells (HEK293). It contains AA Ile 33 - Gln 304 (Accession # B8XCN0). |
| Purity: | 90% |
| Endotoxin Level: | 1.0 EU per μg |
| Format: | Powder |
| Additional Information | |
|---|---|
| Synonyms: | GP,Envelope glycoprotein,GP(1,2) |
| Organism: | Ebolavirus |
| Accn. No.: | ACI28624 |
Sequence Information
Ile 33 - Gln 304
Background
EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.
Product Citations
- Smiley Evans T, Tutaryebwa L, Gilardi KV, Barry PA, Marzi A, Eberhart M, Ssebide B, Cranfield MR, Mugisha O, Mugisha E, Kellermann S, Mazet JAK, Johnson CK (2018) Suspected exposure to filoviruses among people contacting wildlife in southwestern uganda J Infect Dis
Price
378,00 €
