Bambanker Serum Free Cell Freezing Medium for long-term freezing at minus 80°C

Product Description

• Serum-free medium for long-term freezing at - 80°C
• No program freezer and no liquid nitrogen necessary
• Ready to Use (DMSO already included)
• Universal: Common and valuable cell lines from normal and tumor cells can be preserved resulting in a high number of intact cells after thawing
• Recovery rates of even sensitive cells much higher compared to regular media
• Medium has 2 years shelf live at 4°C

One of the most useful techniques in biomedical and biological research is cryopreservation of mammalian cells. The variety of applications for which cultured mammalian cells are suited is virtually limitless. For this reason, one property that is taken advantage of by essentially every lab using cultured cells is their ability to be stored indefinitely at freezing temperatures. In fact, the fear of losing a cell line to contamination or incubator failure is frequently the impetus for making archival storage of cells the first order of business after receiving or generating a new cell line.

While there are numerous different protocols for freezing cells, all involve the use of a ‘freezing medium’ containing a cryoprotectant such as DMSO or glycerol. Once transferred from growth medium to freezing medium, the cells are usually frozen at a controlled rate and stored in liquid nitrogen vapor or at -130°C in a mechanical deep freeze. Although freezing cells is a relatively simple procedure, problems arise when suitable freezers are not available.

This problem has been ressolved by Bambanker Freezing Medium made by LYMPHOTEC, Inc. (Tokyo, Japan) (EU patent EP 1347040 (2003) and sold by BioCat.

The unique feature of Bambanker freezing medium is that it permits cryopreservation of cells at -80°C, obviating the need for an additional and expensive ultra-low freezer and avoiding time consuming and complicated controlled freezing protocols.

Bambanker is a serum-free cryopreservation medium that is delivered ready-to-use and can be kept in the refrigerator for up to two years. Convenient 20 ml bottles are available, making Bambanker freezing medium personally available to individual members of your lab.

The protocol is very simple:

1) Harvest cells in logarithmic growth phase and pellet them by centrifugation

2) Aspirate growth medium

3) Resuspend cells in 1 ml Bambanker (for 0.5 to 10 million cells)

4) Transfer cell suspension to a cryovial and put it in a -80°C freezer. No controlled freezing is required.