mdh (1-312, His-tag) Escherichia coli Protein
| Specifications | |
|---|---|
| Molecular Weight: | 34.9 kDa |
| Tag: | His-tag |
| Source: | E. coli |
| Purity: | > 95 % by SDS - PAGE |
| Activity: | > 450 units/mg.Defined as the amount of enzyme that cleaves 1 umole of oxalacetate and beta - NADH to L - malate and beta - NAD per minute at pH 7.5 at 25°C. Activity Assay : 1. Prepare a 1.45 ml assay bu ffer . The final concentrations are 100 mM Potassium phosphate , 0.13mM beta - nicotinamide adenine dinucleotide, reduced form, 0.25mM oxalacetic acid. 2. Add 50 µl of recombinant MDH protein with various concentrat ions (0. 015 µg, 0. 03 µg ) in assay buffer. 3. Mix by inversion and record the |
| Format: | 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl |
| Additional Information | |
|---|---|
| Organism: | Bacteria |
- Catalog Number
AR50312PU-N-OR - Supplier
OriGene - Size
- Shipping
Price
970,00 €
