Enolase beta (1-434, His-tag) Human Protein
| Specifications | |
|---|---|
| Molecular Weight: | 49 kDa |
| Tag: | His-tag |
| Source: | E. coli |
| Purity: | > 95 % by SDS - PAGE |
| Activity: | > 1.5 units/ml. One unit will convert 1.0 umole of 2 - phosphoglycerate to phospho(enol)pyruvate per minute at pH 7.5 at 25°C. Activity Assay: 1. Prepare 1,450 ul assay buffer. The final concentrations are 81 mM Triethanolamine, 1.9 mM 2 - phosphoglycerate, 0.12 m M beta - NADPH , 25 mM magnesium sulfate, 100 mM potassium chloride, 1.3 mM ADP, 4 unit pyruvate kinase , 6 unit L - lactic dehydrogenase. 2. Add 50 ul of recombinant beta - enolase protein in various concentrations ( 0.25 ug, 0.1 ug, 0.5 ug ) |
| Format: | 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl |
| Additional Information | |
|---|---|
| Synonyms: | GSD13|MSE |
| Organism: | Human |
- Catalog Number
AR50087PU-S-OR - Supplier
OriGene - Size
- Shipping
Price
510,00 €
