SpheroRuler fluorescent calibration beads, 1000 nm, Far-red & Green

Calibration Beads for Super-resolution Microscopy

Product Description

SpheroRuler, 1000 nm, Far-red & Green, is a monodispersed suspension of 1 µm diameter spheres coated with 647 and 488-fluorophores giving a stable blinking in SMLM microscopy. Their consistent size and geometry make them very practical and reliable standards to assess the accuracy of your x-y or z measurements, 3D reconstruction methods or your image quality. Monodispersed and immobile in solution, they can also be used as rulers, for drift correction or as guides to help localize features on your biological samples. 

Features

• Stable blinking - suited for use in SMLM microscopy 
• Consistent size, thoroughly characterized by electron microscopy
• Spherical geometry​ with sharp and thin edges
• Immobile - suitable for drift correction applications
• Bio-compatible​, resuspended in an aqueous buffer and usable along biological samples such as cells or tissue sections
• Easy-to-spot - high fluorescence intensity for practical use as demo or training tools

Compatibility

dSTORM, SRRF, Airyscan confocal, confocal, SEM

SpheroRuler is great for dSTORM calibration experiments because you can use it on your preferred support and buffer. And because you get confident with the new biological structure to image. SpheroRuler also produces simple shapes that are easy to study when you are a dSTORM beginner!

Easy Control of Imaging Process Success

How do you know your imaging process is good with SpheroRuler?  Your beads blink, you reconstruct a ring in 2D and a sphere in 3D experiments, and you check that you retrieve the 1 µm diameter.

SpheroRuler Family

SpheroRuler Calibration Beads are available in different sizes and with different fluorophors:

SpheroRuler calibration beads, 100 nm diameter, Far-red fluorophor (647nm)
SpheroRuler calibration beads, 1000 nm diameter, Far-red fluorophor (647nm)
SpheroRuler calibration beads, 1000 nm diameter, Far-red & Green*
SpheroRuler calibration beads, 1000 nm diameter, Green*  fluorophor (488nm)
SpheroRuler calibration beads, 6000 nm diameter, Far-red fluorophor (647nm)

*Green SpheroRuler beads are also available with FAM fluorophore coating

Supporting Data

Fig.1: One SpheroRuler bead imaged using different microscopy techniques.

Fig. 2: SpheroRuler beads imaged using SRRF-Stream super-resolution microscopy
(A, B). Pictures of SpheroRuler beads acquired in wide-field (A) and SRRF-Stream super-resolution (B) imaging. Scale bar = 5µm (C, D) Local enlargements of A and B pictures respectively. Scale bar = 1µm (E) Fluorescence intensity distributions along the solid lines in C and D. 
Credits: Yao Baoli - Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, 2023