AAV Purification Kit

Product Description

• AAV Purification from cell fraction or media fraction
• Rapid purification within 2 to 4.5 hours
• High AAV recovery, up to 90%
• No specialized equipment needed
• Purification from a variety of AAV serotypes (including AAV9)
• Purify AAV cell culture supernatant from 1 ml to 33.5 ml input per prep
• Purify AAV cell pellet from 1 ml of input per prep
• Yields highly active AAV for in vivo and in vitro experiments
• Up to 33X sample concentration

Norgen provides a range of kits for rapid and simple concentration and purification of AAV vectors from cell lysates and/or cell culture media. These kits feature fast and simple procedures for concentrating and purifying AAV vectors.

Purification is based on spin column chromatography using Norgen’s proprietary resin as an ion exchanger. Contaminating cellular debris is largely removed while contaminating DNA and RNA is reduced using enzymatic digestion. Vectors purified in this manner are highly active for use in in vitro transduction experiments, and are eluted into a small volume for use in downstream applications.

Performance Data