NGS Library Quantification Kit (for Small RNA-Seq)

Product Description

PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations

• Able to quantify NGS Library (Illumina) of a wide spectrum of concentrations, including sub-nanomolar concentrations
• DNA is accurately quantified by using a standard curve constructed from the provided DNA Standards
• Specially designed DNA standards for Small RNA-Seq library; also compatible to NGS library of other molecular weights

Norgen’s NGS Library Quantification Kit (for Small RNA-Seq) offers a PCR-based detection procedure to quantify NGS libraries (specifically Small RNA-Seq) of a wide spectrum of concentrations.  The kit consists of a specially designed primer mix compatible with the Illumina system, that is used in conjunction with the provided 2X Real-Time PCR Master Mix to amplify a library of unknown concentration. The unknown library is accurately quantified by using a standard curve constructed from the provided DNA Standard (range from 20 pM to 2 fM) on a Real-Time PCR System. The kit is specially optimized to quantify Small RNA-Seq libraries with DNA standards that have similar size to a Small RNA-Seq library. However, it could also be used with other types of NGS libraries.

Supporting Data

Figure 1. A representative qPCR baseline graph showing the successful amplification of Quantified NGS Library Standards (Green) with a range from 20 pM to 2 fM, using Norgens NGS Library Quantification Kit (for Small RNA-Seq) (Panel A). Duplicate amplification of a sample Small RNA-Seq library (at 1:10,000 dilution) was performed (Orange). The derived library concentration was 9.41 nM. Norgens NGS Library Quantification Kit (for Small RNA-Seq) is of good quality as shown with the high PCR efficiency and correlation in the standard curve (Panel B) with low background signals (No Template Control NTC as Yellow in Panel A).

Figure 2. Accurate standard curve quantification. Norgen's NGS Library Quantification Kit (for Small RNA-Seq) was compared head-to-head with two competitor's NGS Library Quantification Kits on a Bio-Rad CFX96 Real-Time PCR system. Norgen's kit showed both high correlation and PCR efficiency when compared to thecompetitors'kits.