Phage Display optimized MC1061 F- Electrocompetent Cells (DUOS) > 4 x 10e10 cfu/ug

Product Description

MC1061 F- is a non-amber suppressor strain, identical to SS320 except that it lacks the F′episome. Highly efficient (≥4 × 10e10 cfu/µg) electrocompetent cells are ideal for general cloning or phage display applications.

Please note that as an F- strain, MC1061 F- cannot be used for re-infection by filamentous phage.

Features

• Transformation Efficiency ≥ 4 x 10¹⁰ cfu/µg pUC19
• Control DNA and Recovery Medium included. Recovery Medium is also available separately.
• Packaged as DUOs (2 transformations per tube)

Genotype

F^- araD139 Δ (araA-leu)7697 Δ(lac)X74 galK16 galE15(GalS) lambda- e14- mcrA0 relA1 rpsL150 (Str^R) spoT1 mcrB1 hsdR2

Overview Competent Cells for Phage Display Libraries

*IPTG induction required

Supporting Data

Figure 1. Transformation efficiency of electrocompetent bacterial cells for phage display compared to competitor's specification.