E. cloni 10G (comparable to DH5alpha) Chemically Competent Cells (Subcloning Grade) > 1 x 10e6 cfu/ug

Product Description

Clone, construct libraries or transform Gibson Assembly® reactions all with high-yield, T1 phage-resistant cells in high efficiencies for routine and challenging applications. 

• Direct replacements for standard cloning strains
• Optimised genetics for high yields:
  - Phage T1 resistant
  - Endonuclease and recombination minus
  - Blue/white screening-capable
• High efficiency cells result in high colony counts

The Chemically Competent Cells include Control DNA and Recovery Medium, and are packaged as Subcloning Grade (480ul aliquots for 12 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA.

E. cloni 10G Chemical Competent Cells are also offered as SOLOs and DUOs.

Features

• Transformation Efficiency ≥ 1 × 10⁶ cfu/µg pUC DNA
• Ideal for Gibson Assembly

Genotype

F^- mcrA ∆(mrr-hsdRMS-mcrBC) endA1 recA1 φ80dlacZ∆M15 ∆lacX74 araD139 ∆(ara,leu)7697 galU galK rpsL (Str^R) nupG λ− tonA

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