TruScript First Strand cDNA Synthesis Kit for mRNA

Product Description

This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts).

The kit contains the 2x Reaction Mix and the TruScript™ Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.

Figure 1. Robust Reverse Transcription of All RNA Transcripts. Norgen’s TruScript™ Reverse Transcriptase is a mutant version of Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase that could produce cDNA from different kind of RNA transcript, including those with high difficulty or high degree of secondary structure. Panel A showed that the TruScript™ Reverse Transcriptase was compared with a competitor’s M-MuLV Reverse Transcriptase for RT-PCR of two different RNA transcripts. Norgen’s TruScript™ showed good amplification for both target while the competitor failed to amplify Target #2 with good quantity. Panel B showed that the TruScript™ Reverse Transcriptase was compared with a competitor’s M-MuLV Reverse Transcriptase in an RT-qPCR reaction. Norgen’s TruScript™ Reverse Transcriptase provided a better amplification as shown with a lower Ct value.

Figure 2. High Sensitivity with Robust Amplification from Different Amounts of RNA Input. Norgen’s Norgen’s TruScript™ Reverse Transcriptase was used in an RT-qPCR reaction to amplify the human 5S rRNA transcripts from a dilution series of HeLa total RNA isolated using Norgen’s Total RNA Purification Kit. The PCR target was robustly amplified from a broad range of RNA inputs, from the 10 ng down to 1 pg.