myTags SARS-CoV2 RNA FISH Ready to use Labeled Probe Set detecting full length positive (genomic) strand

FISH probe set for localization of full-length RNA sequence of the 2019 novel coronavirus (SARS-CoV-2) responsible for COVID-19 disease

Product Description

• Pre-labeled ready to use myTags probe sets
• Immortal probe set libraries are available for an unending supply
• The probes are compatible with all cytogenomic applications

The myTags® technology has been expanded to development of FISH probe sets to detect full-length sequence of both the native viral RNA or positive (+) strand and the actively replicating RNA or negative (-) strand from the SARS-CoV-2 virus. The two probe sets were designed to detect both strands in the same hybridization reaction or separate reactions. These probe sets may be used in various FISH applications including human and Vero (African Green Monkey kidney epithelial) cell cultures.

The myTags Expert SARS-CoV-2 probes were designed using genomic information of SARS-CoV-2 strain FDAARGOS_983 (Accn. MT246667.1). These probe sets are specific to the SARS-CoV-2 virus and will not hybridize significantly to any other circulating human coronaviruses, including beta, alpha, and delta coronaviruses. The design strategy used for these probes enables the researchers to use the both sets together, or in separate hybridization mixtures, as probes in each set will not hybridize to one another..

The probe set is provided either as myTags Labeled probes which arrive ready for immediate use in cytogenetic applicationsor as myTags Immortal probe set, which can be amplified for continual use and labeled by the end-user. 

Labeling Options:

• ATTO-488
• ATTO-550
• ATTO-647N

For experiments that need a brighter signal, the use of 3 fluorescent tags is recommended. The brighter signal from the triple tags can assist in detection of lower cellular viral RNA concentrations at earlier experimental time points. For a triple-labeling please order the Triple-label Upgrade in addition. 

Performance Data

Figure 1. Highly Specific Detection of the SARS-CoV-2 Viral Genome. Huh7 cells infected with SARS-CoV-2 and processed 2 days after exposure. (A.) Detection of viral RNA genome using myTags SARS-CoV-2 (+) Strand Labeled Probe Set with ATTO 647N fluorescent tag (magenta). (B.) Detection of SARS- CoV-2 protein present in infected cells with immunocytochemistry (ICC) via antibodies labelled with Alexa 488 fluorescent tag (green). (C.) Signals merged, with cell nuclei visualized in grey/blue via DAPI staining. The co-localization of green and magenta signals demonstrates  the high specificy of the myTags SARS-CoV-2 Labeled Probe.