3rd Generation Lentiviral Packaging Plasmid Mix

For the production of lentiviral particles, three components are generally required:

1. A lentiviral vector containing your inserts of interest (cDNA, shRNA, or miRNA)
2. One or two packaging vectors which contain all necessary viral structure proteins
3. An envelope vector expressing Vesicular Stomatitis Virus (VSV) glycoprotein (G)

The 3rd generation packaging system offers maximal biosafety as the lentiviral Rev gene is supplied as an independent vector from other structure genes, further eliminating the possibility of reverse recombination of vectors into replication competent viral particles.

The 3rd generation lentiviral packaging mix will only support lentiviral expression vectors with a chimeric 5' LTR in which the HIV promoter is replaced with CMV or RSV, thus making it TAT-independent. The 3rd generation lentiviral vectors will not support the production of 2nd generation lentiviral particle productions. All ABM lentiviral vectors are TAT-independent. 

Content and Specifications

Vectors: pLenti-P2A, pLenti-P2B and pLenti-P2C

Selection: Ampicillin (Bacterial Selection) 

Amount: 200 μl (100 µg mixture of pLenti-P3A, pLenti-P3B, and pLenti-P3C)

Appearance: Liquid

Storage: Temperature -20°C or below (shipped at ambient temperature)

Shelf Life: Up to 1 year (when at -20°C or below in a frost-free freezer)