purePlex™ DNA Library Prep Kit with Index Set 4

Speed, Performance, and Auto-Normalization with Unique Dual Indexes

Product Description

seqWell's core technology is a true multiplexing library preparation system for making normalized NGS libraries quickly and easily from a large number of samples. The purePlex DNA Library Preparation Kit leverages seqWell's patented Tn5 transposase-based chemistry where simple molecular tagging steps add dual indexes early on in the library prep process, enabling pooling of samples prior to any bead cleanups or amplification steps.

2.5-hour workflow for 96 samples, 45 min. hands-on time
Auto-normalization of read counts and insert size over 10-fold input range
Early pooling for easier sample handling
Accurate sample identification
purePlex Kits are available with different Index-Sets (384 possible UDIs)

A distinct advantage of the purePlex workflow is the elimination of sample and library normalization which reduces the QC and labor cost compared to traditional UDI workflows.

Recommended Applications

Low-pass whole genome sequencing
Whole small genome sequencing (<50 Mb)
Synthetic construct sequencing (amplicons, plasmids, etc.)

purePlex Library Prep Workflow

Pureplex Workflow Diagram 1200x1000 1 1024x776

Kit Content

i7 Tagging Reagent Plate
i5 Tagging Reagent Plate
Coding Buffer (3X)
X Solution
MAGwise Paramagnetic Beads
Normalization Reagent
Library Primer Mix

Supporting Data

Auto-normalization of Insert Size

Pureplex Insert Size Fig1 A 700x500 2

Pureplex Insert Size Fig1 B

Pureplex Insert Size Fig1 C

Fig.1. The insert size within a pool of samples is consistent regardless of input (panel A, left) or GC content (panel B, lower left). In contrast, Nextera XT libraries have varied fragment distributions from GC content even after normalizing the sample input.

Auto-normalization of Read Depth

Pureplex Fig.2 Dna Input Titration 700x500 1

Fig.2. Samples were normalized to inputs of 3, 5, 10, and 30 ng then underwent purePlex library prep with (+) and without (–) normalization reagent. Read counts for each sample are equal, regardless of input, when normalization reagent is used. In contrast, without normalization reagent, sample read count scales with input.