CUTANA Quick Cleanup DNA Purification Kit

Paramagnetic Bead-based Purification - Compatible with 8-strip Tubes

Product Description

The CUTANA™ Quick Cleanup DNA Purification Kit can be used to purify CUT&RUN or CUT&Tag DNA and remove unwanted adapter-/primer-dimers from these reactions. Unlike many kits that purify DNA using spin columns, this kit uses SPRI paramagnetic beads for streamlined, higher throughput workflows easily integrated into CUTANA™ genomic mapping assays. Depending on the application, various SPRI bead:DNA ratios are added to capture target DNA.

In CUT&RUN, pAG-MNase cleaves DNA proximal to antibody-labeled chromatin, releasing it into the supernatant. It is imperative to recover the highest DNA yield possible from the supernatant; therefore, a higher bead:DNA ratio is added to span a wide range of DNA fragment lengths. This kit includes sufficient volume to perform 48 total CUT&RUN reactions at an empirically optimized higher bead ratio.

In CUT&Tag, pAG-Tn5 cleaves antibody-bound chromatin and simultaneously ligates adapter DNA, resulting in longer DNA fragments. Further, in the EpiCypher CUTANA™ Direct-to-PCR CUT&Tag workflow, indexing PCR is used to amplify next-generation sequencing (NGS) libraries directly from the reaction mixture prior to DNA purification. In this case, a lower bead ratio is ideal for preferable enrichment of longer DNA fragments and selective cleanup of sequencing libraries. This kit includes sufficient volume to perform 96 total CUT&Tag reactions at an empirically optimized lower bead ratio.

This kit can also be used to cleanup NGS libraries by removing contaminating small fragments such as adapter dimers in CUT&RUN (~150 bp) and primer dimers in CUT&Tag (~25-75 bp).

Validation Data

Figure 1: CUT&RUN DNA fragment size distribution analysis.
CUT&RUN was performed as described in Figure 2. CUT&RUN DNA was purified using the CUTANA™ Quick Cleanup DNA Purification Kit. Purified CUT&RUN DNA was used to prepare NGS sequencing libraries that were analyzed by Agilent TapeStation®. This analysis confirmed that the CUTANA™ Quick Cleanup DNA Purification Kit successfully enriched mononucleosomal-sized DNA in CUT&RUN (~300 bp peaks represents 150 bp nucleosomes + sequencing adapters).