Whether you’re using microRNAs to study cellular processes or develop the next generation of therapeutics, SBI’s comprehensive collection of human and mouse precursor microRNA lentivectors are a superior alternative to synthetic microRNAs. Like synthetic microRNAs, SBI’s human and mouse pre-microRNA expression constructs can be transfected into target cells for transient microRNA expression. But unlike synthetic microRNAs, they can also be transduced into a variety of target cells—including primary cells, stem cells, and other hard-to-transfect cell lines—to create stable microRNA-producing cell lines, maximizing your options for microRNA expression.
Pooled Human Lenti-miR Libraries for efficient microRNA screening as well as Human and Mouse pre-microRNA Scramble Negative Controls are also available.
SBI’s Lenti-miRs are designed for efficient expression and accurate processing into mature microRNAs, for native microRNA-like behavior. Each construct is cloned with the native stem-loop structure plus an additional 200–400 bps of upstream and downstream DNA, ensuring that the microRNA processing machinery operates just as it would with the native transcript.
Pre-microRNA Expression Lentivectors use the native microRNA processing machinery.
The precursor microRNA expression lentivectors drive expression of the microRNA precursor with the constitutive CMV promoter, for strong expression in common cell types including HeLa, HEK293, and HT1080 cell lines. Transductants/transfectants can be easily selected for or sorted using copGFP or puromycin markers.
Leveraging SBI’s powerful and well-regarded third-generation lentivector technology, each of their miRZip lentivectors expresses a short hairpin RNA (shRNA) that, after processing, preferentially produces an anti-sense microRNA. The hairpin is rationally designed to be asymmetric, ensuring that the sense strand does not contain the endogenous microRNA sequence and enabling accumulation of the anti-microRNA. The result is robust derepression of the transcripts targeted by the microRNA being “zipped,” and elevation of the corresponding protein levels.
In addition, a miRZip Pooled anti-microRNA Knockdown Library for high-throughput phenotypic screening as well as a miRZip anti-microRNA Scramble Negative Control are offered.
miRZip shRNAs produce short, single-stranded anti-microRNAs that competitively bind their endogenous microRNA target and inhibit its function.