When you’d like to profile miRNA with qPCR, SBI’s QuantiMir Kit can get your samples ready. By efficiently converting all of your miRNA into cDNA, you can get accurate, sensitive, and quantitative qPCR data on the miRs in your sample. Great for understanding differential miRNA expression, simultaneously profiling siRNA and mRNA, and more.
Each QuantiMir Kit includes three reference assays - human U6, mouse U6 and human/mouse miR-16. We also offer reference assays as individual 50-assay kits.
The QuantiMir Kit enables robust miRNA quantitation through a simple and efficient workflow. Highly efficient poly-A tailing and reverse transcription in a single reaction tube provides uniform cDNA synthesis of miRNAs. The optimized reaction conditions and buffer components maximize cDNA yield when starting with several micrograms down to picograms of input total RNA. The universal 3′ tag sequence incorporated during reverse transcription enables easily scalable and accurate miRNA expression analysis by qPCR - profile thousands of different miRNAs from a single reverse transcription reaction.
The result is a cDNA pool of anchor-tailed miRNAs that are ready for qPCR.