X-CLARITY Tissue Clearing System
Tissue Clearing for High-resolution 3D Imaging
Clearing – Labeling – Imaging
X-CLARITY™ is based on the CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging / Immunostaining / In situ-hybridization-compatible Tissue hYydrogel) method. With CLARITY, preserved tissues are embedded in a hydrogel matrix and lipids are actively extracted through electrophoresis to create a stable and optically transparent tissue-hydrogel hybrid that is chemically accessible for multiple rounds of antibody labeling and imaging. Native cytoarchitecture remains intact and even endogenous fluorescent proteins are preserved for robust fluorescence imaging.
Key Features of the X-CLARITY Tissue Clearing System
- Collection of systems and ready-to-use reagents
- Simplified and standardized tissue clearing
- Rapid, safe, efficient and reproducible
- Non-destructive
- Endogenous fluorescent protein expression kept intact
- Adaptable for various tissues
Components of the X-CLARITY Tissue Clearing System
The CLARITY Tissue Clearing system represents a collection of ready-to-use reagents and dedicated instrumentation. It clears a variety of tissues and organs in a simple and standardized manner.
Every component plays a specific role in the clearing process.
Hydrogel Infusion
A fixed tissue sample is incubated in the X-CLARITY™ Hydrogel Solution Kit to allow hydrogel monomers to diffuse uniformly throughout the sample.
X-CLARITY™ Polymerization System
Monomers polymerize in the anaerobic environment, linking the biomolecules to a hydrogel network, which preserves molecular information and structural integrity. This step is facilitated by the use of the X-CLARITY™ Polymerization System.
X-CLARITY™ Tissue Clearing System II
Once the tissue-hydrogel hybrid has been formed, the hybrid is cleared in the X-CLARITY™ Tissue Clearing System II using the ready-to-use Electrophoretic Tissue Clearing Solution. Lipids are extracted actively through electrophoresis or passively, leaving behind a stable and transparent tissue-hydrogel hybrid that is chemically accessible for molecular phenotyping.
Performance
Whole Thy1-YFP Mouse Brain
The Thy1-YFP signal is preserved and vibrant after tissue clearing with the X-CLARITY™ systems and reagents. Courtesy of Hyung Jin Choi, PhD Seoul National University College of Medicine.
Mouse Embryo
Mouse embryo (E 21.5) cleared with the X-CLARITY™ systems and reagents. Courtesy of Michal Shoshkes-Carmel, PhD and Andrea Stout, PhD, University of Pennsylvania.