Genome-wide CRISPR/Cas9 Gene Knockout

Human, Mouse and Rat CRISPR/Cas9 Gene Knockout Constructs

CRISPR Gene Knockout sgRNA vectors and viruses are highly effective at achieving knockout of your target gene. Cas9 functions to create a double-stranded break within an early exon triggering repair via Non-Homologous End-Joining (NHEJ) mechanism resulting in deleterious frameshift mutations. A comprehensive genome-wide collection of All-in-One (spCas9 and sgRNA expressing) and sgRNA only expressing constructs targeting any human, mouse, or rat gene is offered.

• Lentiviral Human, Mouse and Rat CRISPR/Cas9 Gene Knockout Constructs
• AAV Human, Mouse and Rat CRISPR/Cas9 Gene Knockout Constructs
• Non-Viral Human, Mouse and Rat CRISPR/Cas9 Gene Knockout Constructs

sgRNA CRISPR/Cas9 All-in-One Lentivector Sets

Gene-specific sets of three sgRNA expression constructs designed to guide Cas9 to cleave exonic gDNA resulting in frameshift mutations and ultimately gene knockout are provided in the lentivector pLenti-U6-sgRNA-SFFV-Cas9-2A-Puro.

sgRNA CRISPR/Cas9 All-in-One AAV Vector Sets

Gene-specific sets of three sgRNA expression constructs designed to guide saCas9 to cleave exonic gDNA resulting in frameshift mutations and ultimately gene knockout are provided in the AAV vector pAAV-PGK-saCas9-U6-sgRNAsa-hGH-amp.

sgRNA CRISPR/Cas9 All-in-One Non-Viral Vector Sets

Gene-specific sets of three sgRNA expression constructs designed to guide Cas9 to cleave exonic gDNA resulting in frameshift mutations and ultimately gene knockout are provided in the non-viral vector pNV-sgRNA-Cas9-2A-GFP.