Exosome Isolation: SmartSEC Purification

Choose from:

• SmartSEC™ Single

• SmartSEC™ Mini EV Isolation System

• SmartSEC™ HT EV Isolation System

• SmartSEC-DeLipo™

Unlock uncompromised extracellular vesicle isolation whether you’re working with a mere 10 µL of precious developmental-biology fluid or screening 96 clinical samples in under an hour.

SmartSEC combines a proprietary resin with contaminant-trapping chemistry and ready-to-use spin-column or 96-well formats to deliver faster, higher-purity, and more reproducible Exosome isolations. From single-drop inputs to high-throughput workflows, it consistently outperforms standard SEC’s slower, co-elution-prone protocols.

Why SmartSEC Outperforms Standard SEC

• Proprietary Resin with Contaminant Trapping
  Unlike conventional SEC, which often co-elutes abundant plasma proteins and lipoproteins with EVs, SmartSEC incorporates a custom resin plus a “contaminant trapping” feature that selectively retains non-EV components—delivering cleaner fractions in a single step.
• Optimized Formats for Every Scale
  Mini (Spin-column): Pre-loaded columns calibrated for as little as 10 µL of biofluid—no packing required—ideal for precious or low-volume samples.
  Single (Spin Column); Pre-loaded columns calibrated for as little as 100 µL of biofluid—no packing required—ideal for quick and easy exosome isolation.
  HT (96-well plate): Pre-aliquoted resin in a filter plate lets you process 1–96 serum/plasma samples in under an hour with no plate prep or manual fraction collection.
• Faster, Easier Workflows
  Load your sample, incubate (≈30 min for Mini; plate-wide for HT), then centrifuge twice to recover highly pure EVs—versus standard SEC’s multi-hour runs and fraction pooling.
• Superior Purity, Yield & Reproducibility
  Gentle SEC separation plus contaminant trapping helps outperform ultracentrifugation and traditional SEC in both particle recovery and protein removal—ensuring reproducible results across labs and sample types.

Compatible Across Sample Types

• Plasma/Serum: Clean, reproducible EV fractions suited for liquid biopsy and biomarker discovery.
• Cell Culture Media: Consistent EV isolation for mechanistic studies and method development.

Go Even Further with SmartSEC-DeLipo™

Even with the best SEC methods, lipoproteins can remain a stubborn contaminant in plasma- and serum-derived EV preps. SmartSEC-DeLipo™ available as a standalone column or paired seamlessly with SmartSEC—selectively removes lipoproteins that co-elute with extracellular vesicles. The result: ultra-pure, lipoprotein-depleted EV fractions that maximize confidence in downstream RNA, proteomics, and biomarker analyses.

• Sample Volume: 100–250 µL of biofluid (serum, plasma, hemolymph, apoplastic fluid, culture media)
• Format: Spin-column pre-loaded with SmartSEC resin, collection tubes, and buffer for 10 reactions
• Performance:
  • Higher purity and yield than ultracentrifugation
  • Preserves EV integrity
• Applications: Mass spectrometry, Western blotting, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), RNA-Seq

• Sample Volume: 10–100 µL of biofluid (serum, plasma, hemolymph, apoplastic fluid, culture media)
• Format: Spin-column pre-loaded with SmartSEC resin, collection tubes, and buffer for 10 reactions
• Performance:
  • Higher purity and yield than ultracentrifugation
  • Preserves EV integrity
• Applications: Mass spectrometry, Western blotting, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM)

• Throughput: Up to 96 samples in a single 96-well plate run in < 1 hour
• Sample Volume per Well: 250–500 µL serum or plasma; partial-plate runs possible, unused wells remain sealed
• Features:
  • No pre-treatment of plasma required
  • Compatible with manual and automated liquid handlers
  • Purity/yield superior to ultracentrifugation and competitor SEC columns
• Applications: EV biomarker discovery, diagnostics, therapeutics R&D

The SmartSEC-DeLipo™ kit takes extracellular vesicle isolation further than ever before. By uniting SmartSEC’s advanced mixed-mode chromatography with dedicated lipoprotein removal, it eliminates one of the biggest sources of noise in blood-derived EV preps. The result: cleaner fractions that reveal the true biological signals, free from lipoprotein-bound microRNAs and other confounders.

With a streamlined workflow, high yield, and compatibility across plasma, serum, breast milk, and more, SmartSEC-DeLipo delivers ultra-pure, lipoprotein-depleted sEVs ready for RNA, proteomics, and functional assays. Available in both column and 96-well HT formats, it ensures reproducibility from a single sample to entire cohorts—accelerating discovery and meeting the most demanding standards of rigor.