Site-specific Chromatin Loop Formation by dCas9

CLOuD9 for Chromatin Loop Reorganization using CRISPR-dCas9

• This innovative CLOuD9 System is only sold by SBI
• Leverage the simplicity of CRISPR/Cas9 targeting—free of DNA cleavage activity—for inducible and reversible chromatin loop formation anywhere in the genome
• Chromatin loop formation and the resulting changes in gene expression across multiple loci and cell lines is amply demonstrated in Morgan, et al,
• Great for studying the role of chromatin in the control of gene expression in a range of areas, including:
  - Developmental biology
  - Cancer biology
  - Chromatin structural biology
  - Chromatin-based therapeutic strategies

Available only from SBI and featured in a recent Nature Communications article, our CLOuD9 Gene Expression Regulation Kit gives you an exciting and innovative tool for studying—and potentially controlling—gene expression regulation by putting you in control of chromatin loop formation. Gain a clearer understanding of the dynamic effects of chromatin restructuring on the expression of your gene-of-interest and increase gene expression in certain cellular contexts. Combining the simplicity of CRISPR/Cas9 targeting—free from DNA cleavage activity—with inducible and reversible protein dimerization partners, the CLOuD9 Gene Expression Regulation Kit adds a new dimension to your gene regulation studies.

Chromatin Loop Formation with CLOuD9

Figure 1. The CLOuD9 System enables formation of inducible and reversible chromatin loops anywhere in the genome.