Subcellular Localization Vectors

Tools for fluorescence labeling of subcellular structures

Subcellular localization vectors express a fluorescent protein fused to a specific protein tag directing the fusion protein to the desired subcellular location.

Molecular trafficking is a dynamic process in eukaryotic cells and the Cyto-Tracers provide the ability to light up cell compartments to monitor movement and localization of organelles and to trace endocytosis and exocytosis. Our partner SBI has created a line of lentivector-based Cyto-Tracers that utilize GFP-fusion proteins to mark cellular compartments, organelles, vesicles and structures to enable more long-term and more in-depth experimentation. The Cyto-Tracers can be used in transfections as well as packaged into virus to create stable copGFP or RFP tracer cell lines in primary cells, tumor cell lines and stem cells.

• Stable lentivector-based system
• Great for creating stable reporter cell lines
• Ideal for co-localization studies
• Monitor cellular dynamics and functional studies in real time
• Create your own subcellular label for a broad range of studies

How it works

Target Locations

Organelles - Endoplasmic reticulum, endosomes, Golgi apparatus, ER-Golgi vesicles, lysosomes, membrane, mitochondria, peroxisomes, autophagosomes

Exosomal Proteins - CD9 Tetraspanin, CD63 Tetraspanin, CD81 Tetraspanin

Cytoskeletal and adhesion proteins - β-actin, α-tubulin, microtubules

Nuclear Proteins - Histone H2B

Other - Cytosol, plasma membrane, inner leaflet membrane, dendritic membrane

Tracking of subcellular activities

Cyto-Tracers mark your compartment of choice.