Subcellular Localization Vectors

Tools for fluorescence labeling of subcellular structures

Subcellular localization vectors express a fluorescent protein fused to a specific protein tag directing the fusion protein to the desired subcellular location.

• Easy labeling of cell compartments and proteins
• No exogenous chemical compounds or cofactors required
• Wide range of fluorescent colors from Evrogen: TagBFP, TagCFP, TagGFP2, TagYFP, TagRFP, FusionRed, mKate2, TurboGFP, TurboRFP, TurboFP602
• Lentivector-based green or red fluorescent Cyto-Tracers from SBI, available as plasmid DNA or pre-packaged pseudoviral particles
•  Overview of Basic Fluorescent Proteins

Ready-to-use expression vectors are available for labeling of:

Organelles

Endoplasmic reticulum, endosomes, Golgi apparatus, lysosomes, membrane, mitochondria, peroxisomes

Cytoskeletal and adhesion proteins

β-actin, α-actinin, VE-cadherin, EB3 protein, microtubules, talin, α-tubulin

Gap Junction Proteins

Connexin 43

Vesicular Transport Proteins

Clathrin LCB

Nuclear Proteins

Histone H2B, Lamin B1

Signal Transduction Proteins

Annexin, CD151, CDC42, paxillin, Rab-5A

Metabolic Pathway Proteins

PDHA1

Fluorescent microscopy of mammalian cells expressing Evrogen fluorescent proteins targeted to the different subcellular structures and proteins.