The 3-D Life  Biomimetic Hydrogel System

The 3-D Life technology is an integrated system for the generation of biomimetic hydrogels used in 3-D cell culture. The 3-D Life system consists of two polymers that form a hydrogel by covalent crosslinking at ambient temperature. The chemical reaction is non-toxic to cells and occurs rapidly, preventing cell settlement before the gel is formed.

• Defined composition of biologically inert synthetic polymers and biopolymers
• User-controlled biomimetic modifications (e.g. peptides, proteins)
• Robust gel formation and handling
• Wide range of ligand density (up to 5 mmol/l)
• Tunable gel stiffness
• Protease-free dissolution of gels for easy cell recovery

Setup of Biomimetic Hydrogels

The setup of hydrogels is very simple and does not require specific equipment or cell-harming treatments. Biomimetic modifications (e.g. covalent attachment of adhesion peptides) can be performed right before gelation occurs. The modular system makes it easy to modify gels with biomimetic factors as desired for each experiment.

Mix and Match to setup gels of defined properties

Thiol-reactive polymers (functional PVA or dextran) can be combined with thiol-containing crosslinkers (either PEG-link or CD-Link or HyLink) for gels with different properties.

3-D Life Biomimetic Hydrogels are available as

• Ready-to-go hydrogel kit with preconfiguered composition
• Flexible ready-to-design kits for slow gelling hydrogels (SG versions)
• Flexible ready-to-design kits for fast gelling hydrogels (FG versions)
• All components are also available seperately