1-Step probe-based RT-qPCR Systems enable detection and relative quantification of RNA expression levels using a one-step real-time RT-qPCR method, combining Reverse Transcriptase and Taq qPCR Master Mix in a single reaction well.
The Probe-based qPCR approach allows multiple targets to be quantified in a single reaction (multiplexing) by using a unique fluorescent dye for each amplicon-specific probe.
The SensiFAST™ Probe One-Step Kits use a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR. This also gives SensiFAST Probe One-Step unbeatable efficiency in multiplexing.
To find the optimal SensiFAST kit for your real-time PCR instrument and application, you can also use this Real-Time PCR Selection Chart.