VividFISH^™  FISH Probes from GeneCopoeia

Bright fluorescent probes for disease gene-specific detection and chromosome enumeration

VividFISH Disease Gene FISH Probes

• Exceptionally bright fluorescent probes make chromosome detection easy and convenient
• Disease-specific Probes are contained in mixes for fluorescence in two colors (green and orange)
• Pretreatment kit for FFPE samples also available

GeneCopoeia´s VividFISH™ fluorescence in situ hybridization (FISH) locus-specific probes are the ideal tools for disease gene detection. FISH gene-specific probes are extremely valuable for diagnosis and/or prognosis of disease with chromosomal abnormalities, gene amplification, or gene depletion.

Performance Data

Fig.1 VividFISH^TM Probe for Human ALK break apart. H2228 cell showing the ALK translocation (left, arrows) and lung cancer cells from a formalin fixed, paraffin-embedded (FFPE) sample that show normal ALK distribution (right) where probed with the VividFISH ALK break apart set. The 5‘ end of ALK is labeled with the green probe, and the 3‘ end of ALK is labeled with the orange probe. DNA (blue) is stained with DAPI.

VividFISH Chromosome Enumeration (CEP) FISH Probes

• Exceptionally bright fluorescent probes make chromosome detection easy and convenient
• CEP probes are available with green or orange fluorescent lable
• Great for use in chromosome copy number determination for genome editing applications
• Pretreatment kit for FFPE samples also available

GeneCopoeia´s VividFISH™ fluorescence in situ hybridization (FISH) chromosome probes are the ideal tools for chromosome detection. FISH chromosome probes are extremely valuable as:

• Control probes to accompany disease gene-specific (LSI) FISH probes
• Chromosome enumeration probes, to be used to determine chromosome copy number in diagnosis and prognosis of cancers and other diseases
• Chromosome enumeration probes for determining target chromosome copy number in CRISPR- and TALEN-mediated genome editing projects

Performance Data

Fig.2 VividFISH™ green CEP probe for chromosome 7. Diploid human cell line HT1080 was probed with centromere sequences carrying green fluorescent lable. DNA (blue) is stained with DAPI.

Fig.3 VividFISH™ orange CEP probe for chromosome 7. Hypotriploid human cell line HEK293 was probed with centromere sequences carrying orange fluorescent lable. DNA (blue) is stained with DAPI.