Transcription Factor Reporter Vectors

Transcription factor reporter vectors for monitoring signalling pathway activity

These vectors contain a reporter gene, such as luciferase or a fluorescent protein, placed downstream of a promoter with binding sites for a specific transcription factor. You use them to measure the transcriptional activity of a pathway of interest in live cells, converting signalling events into a quantifiable optical readout. They are commonly applied in drug screening and pathway characterisation studies, where changes in reporter expression indicate activation or inhibition of the target transcription factor. Assays can be run in transient transfection or stable cell line formats depending on the experimental design.