Plasmid prep, PCR clean-up, and normalisation kits for high-quality Sanger sequencing templates
When you prepare templates for Sanger sequencing reactions, template quality drives read length and chromatogram quality. This section covers high-quality plasmid prep kits, PCR product clean-up reagents, and template normalisation tools.
Pick by template type and throughput.
- Template type. Plasmid versus PCR product versus genomic. Each has appropriate clean-up chemistry.
- Clean-up. Column-based for cleanest template. Magnetic-bead for high-throughput.
- Normalisation. For consistent Sanger read length normalise template concentration before sequencing; over- or under-loaded reactions both fail.
- Caveat. Match template prep to your facility's typical workflow rather than mixing across kits.
AdvancedSeq supplies template prep reagents in this inventory.
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