Stellaris RNA FISH hybridisation and wash buffers for consistent, specific signal
Pre-formulated hybridisation and wash buffers control hybridisation stringency, signal-to-background, and probe-specific signal intensity in Stellaris RNA FISH protocols. Quality and lot consistency drive reproducibility.
Match to protocol.
- Standard DesignReady protocol. Use the standard hybridisation and wash buffer set.
- Tissue-clearing protocols. May require modified buffer compositions. Check supplier guidance.
- Lot consistency. For long-running studies lock to specific lots.
- Substitution. Use the manufacturer's matched buffer set; substituting buffers from other vendors degrades signal predictably.
LGC Biosearch supplies the matched buffer set.