SNAP-ChIP Spike-in Panels & Validated Antibodies

SNAP-ChIP spike-in panels for validating ChIP antibody specificity against off-target binding

When you want to validate ChIP-grade antibody specificity, SNAP-ChIP spike-in panels are mixtures of modified recombinant nucleosomes with defined PTM combinations. Adding the spike-in to your IP reveals off-target binding to non-cognate modifications in your real samples. Run a SNAP-ChIP calibration before scaling any chromatin profiling experiment.

Pick panel by experiment.

1. Methylation panels. For methylation-mark mapping.
2. Acetylation panels. For acetylation-mark mapping.
3. Combinatorial panels. For mixed PTM combinations.
4. Validated antibody catalogue. Covers the major histone modifications. Off-catalogue antibodies must be validated independently.
5. Use. Run SNAP-ChIP spike-in calibration first for any chromatin profiling experiment that will be published or scaled. Antibody specificity is the single biggest source of irreproducibility in the field.

EpiCypher supplies SNAP-ChIP.