siRNA Constructs

siRNA constructs for dose-titratable gene knockdown from transient to stable

When you want partial gene knockdown rather than complete knockout, siRNA constructs deliver the dose-titratable phenotype. Synthetic siRNA oligos give you 3- to 7-day transient knockdown; lentiviral and AAV vector sets give you weeks-to-months stable knockdown. siRNA, shRNA, and CRISPR knockout sit alongside each other: knockdown when partial loss-of-function tells you more than a clean null, CRISPR when you need the full null.

Decide duration first, then format.

1. Duration. For 3- to 7-day transient knockdown choose synthetic siRNA oligo transfection. For stable knockdown choose lentiviral or AAV vector-encoded shRNA or siRNA. For inducible knockdown choose a Tet-on vector.
2. Construct count. Pooled vector sets containing four siRNAs per gene outperform single constructs for reliability and reduce off-target risk through sequence diversity. Start with a pooled set for unvalidated targets.
3. Delivery. For routine HEK293 or HeLa use lipid transfection. For primary T cells, neurons, or pluripotent stem cells use lentiviral or AAV.
4. Species. Human, mouse, and rat coverage is standard.
5. Validation. Verify knockdown depth by qPCR or Western before downstream phenotypic work. Catalogued knockdown efficiencies are starting points, not guarantees.

ABM carries siRNA Lentivector Sets and AAV Pooled Vectors across human, mouse, and rat for tens of thousands of genes. For shRNA libraries at screen scale, Cellecta.