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Sample Loading & Capillary Electrophoresis

Polymers, buffers, and consumables for capillary electrophoresis on ABI sequencing instruments

When you run ABI capillary instruments for Sanger sequencing or fragment analysis, this section covers POP-4 and POP-6 polymers, running buffers, and sample-loading consumables. Polymer quality and lot consistency drive CE separation quality.

Pick polymer by application and instrument.

  1. Polymer choice. POP-4 for fragment analysis with separation of short fragments. POP-6 for higher-resolution Sanger sequencing.
  2. Instrument compatibility. Each polymer matched to specific ABI instrument models (310, 3130, 3500, 3730).
  3. Caveat. Use the polymer specified by the manufacturer; mismatched polymer degrades separation quality.
  4. Storage. Verify storage stability.

AdvancedSeq is the supplier in this inventory.

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