Recombinant Nucleosomes (rNucs)

Recombinant nucleosomes as defined chromatin substrates for structural and biophysical studies

When you want unmodified recombinant nucleosomes as defined chromatin substrates, this section covers them. Histone octamers assembled in vitro around 601 Widom positioning DNA give homogeneous biophysically defined substrates for in vitro biochemistry, biophysics, and structural biology.

Pick by histone and DNA context.

1. Histone composition. Canonical core versus variant histones (H2A.Z, H3.3, CENP-A, macroH2A). Variant context matters for some enzymes.
2. DNA length and sequence. 601 positioning sequence standard. Tetra- or oligo-nucleosome arrays for higher-order context.
3. Labelling. Biotinylation or fluorescent label for bead-based or imaging assays.
4. Use case. Single-positioned 601 for mechanistic enzyme studies (controls substrate variability). Oligonucleosome arrays for higher-order context.
5. Storage. Verify freeze-thaw tolerance.

EpiCypher carries the rNuc catalogue in this inventory.