Promoter reporter clones for quantitative luciferase and GFP readouts of gene regulation
When you want to measure how a specific promoter responds to drugs, signalling perturbations, transcription-factor over-expression, or disease-mimicking conditions, promoter reporter clones give you the quantitative readout. The construct places a defined human, mouse, or rat genomic promoter sequence upstream of a luciferase, GFP, or dual-reporter gene.
Pick by promoter coverage and assay format.
- Promoter coverage. Verify your gene of interest is represented before ordering. GeneCopoeia covers tens of thousands of human, mouse, and rat promoters.
- Reporter format. For secreted-reporter kinetic time-course choose Gaussia luciferase. For endpoint signal choose firefly. For dual-reporter internal normalisation choose the dual-reporter format.
- Vector backbone. For transient assays choose pEZX-PG variants. For lentiviral integration into stable reporter lines choose pEZX-LvPF02.
- Insert length. Proximal 1-2 kb promoter is standard. For longer cis-regulatory regions check availability before ordering.
- Controls. Always run a parallel empty-vector control to rule out non-specific reporter response.
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