Pluripotency & Differentiation Analysis

Pluripotency reporters and differentiation assays for stem cell characterisation and lineage tracking

When you need to characterise pluripotent stem cells or track lineage commitment during differentiation, this section covers reporters and assays. Pluripotency-promoter reporter lentivirus (Oct4, Nanog, Sox2), lineage-specific differentiation reporters, and matched antibody and qPCR primer sets.

Pick by marker and readout.

1. Core pluripotency. Oct4, Nanog, Sox2 minimum. Expanded panels add lineage commitment markers.
2. Format. Fluorescent for FACS. Luciferase for population kinetics. Antibody and qPCR for endpoint.
3. Vector type. Lentiviral for stable integration. Non-integrating episomal for transient detection.
4. Live versus endpoint. Live-cell tracking with reporters. Endpoint with antibodies and qPCR.
5. Caveat. Reporter signal can persist after gene silencing. Combine with qPCR confirmation for iPSC line characterisation.

SBI System Biosciences supplies pluripotency reporter vectors and lineage-tracking systems.