PiggyBac transposon system for footprint-free integration of large genetic payloads
When your transgene is too large for lentivirus or you need footprint-free engineering, PiggyBac transposase-mediated integration handles payloads up to 100+ kb and can be re-excised cleanly. Useful for cell-line engineering, iPSC reprogramming, and translational work with very large constructs.
Pick by payload and excision needs.
- Payload size. PiggyBac shines beyond ~10 kb where lentivirus packaging fails.
- Excision. Excisable PiggyBac for transient or reversible integration. Integrating-only variants for stable expression.
- Transposase delivery. Plasmid co-transfection versus mRNA versus stable transposase cell line. mRNA gives transient expression with cleanest excision.
- Selection compatibility. Match marker to your selection scheme.
- Verification. For single-copy integration use ddPCR or Southern. PiggyBac integrates randomly; verify integration count and site for quantitative work.
SBI System Biosciences leads the PiggyBac platform and matched donor vectors.
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