Modified Designer Nucleosomes (dNucs)

Modified designer nucleosomes with defined histone PTMs for chromatin enzyme studies

When you want to study a writer, reader, or eraser enzyme on its real chromatin substrate, dNucs give you a defined nucleosome bearing the specific modification at the right residue. The technology pairs purified recombinant histones bearing PTMs (H3K4me3, H3K9me3, H3K27me3, H3K27ac, H3K36me3, others) with 601 Widom positioning DNA.

Pick by what you need to install.

1. Modification. dNucs are available bearing single modifications and combinatorial modifications. Choose the mark matching your target enzyme or antibody.
2. Symmetry. Standard dNucs carry the mark on both modified-histone copies. For asymmetric work check availability.
3. Histone variant context. Canonical core is the default. If your biology cares about variant histones (H2A.Z, H3.3, CENP-A, macroH2A) choose dNucs assembled with the variant.
4. DNA context. 601 sequence is standard for cleanest kinetic data. For higher-order chromatin use oligo-nucleosome arrays.
5. Label. For bead-based or homogeneous assays choose biotinylated or fluorescent-labelled. For free substrate use unlabelled.

EpiCypher carries the dNuc catalogue. For drug screening on chromatin modifiers use nucleosome substrates rather than peptides; peptide-only screens routinely produce hits that fail when retested on nucleosomes.