Minicircle DNA Vector Technology

Minicircle DNA vectors for backbone-free, high-level transgene expression in mammalian cells

When you need backbone-free DNA constructs (no bacterial selection or replication elements) for higher and longer transgene expression in mammalian cells, minicircle DNA delivers it through intramolecular recombination from parental plasmids. Used for transient over-expression, vaccine development, and gene therapy research.

Pick by production scale.

1. Production method. In vivo recombinase-based production from parental plasmid. Some kits supply the recombinase E. coli system. Others provide parental plasmid and recombinase ready-mixed.
2. Scale. Scale parental plasmid to 50 mL or larger culture for high-yield minicircle production. Recombinase induction efficiency limits low-scale yield.
3. Downstream application. Transient expression, vaccine, in vivo gene therapy each have specific design considerations.
4. Storage. Verify storage stability.

SBI System Biosciences supplies minicircle production kits.