microRNA Overexpression & Knockdown Constructs

MicroRNA overexpression and knockdown vectors using lentiviral precursor and sponge constructs

When you want to over-express a specific miRNA (precursor cassette expression) or inhibit an endogenous miRNA (sponge constructs or antisense vectors), this section covers the vectors. Lentiviral backbones are standard.

Pick by gain or loss-of-function design.

1. Over-expression. Precursor cassette in a lentiviral expression vector. Match miRNA promoter context.
2. Loss-of-function. Sponge vectors give cleaner phenotypes than antisense oligonucleotides because of sustained expression.
3. Validation. Lot-level functional knockdown or over-expression data should be available.
4. Selection marker. Match to your downstream selection scheme.
5. Inducibility. Tet-on for time-sensitive perturbation.

SBI System Biosciences supplies miRNA modulation vectors.