Methylated RNA Immunoprecipitation (MeRIP)

MeRIP kits for transcriptome-wide m6A methylation mapping by sequencing or qPCR

When you want transcriptome-wide m6A methylome mapping, MeRIP enriches m6A-methylated RNA fragments using anti-m6A antibodies for downstream sequencing or qPCR. The workhorse for m6A epitranscriptomics research.

Pick by antibody and input.

1. Antibody quality. m6A antibody specificity varies. Validated antibodies prevent false positives.
2. Input. Standard MeRIP needs microgram-scale RNA. Low-input variants emerging.
3. Spike-in controls. Synthetic methylated and unmethylated RNA controls enable quantitative comparison across samples. Essential for quantitative m6A profiling.
4. Downstream readout. Sequencing for transcriptome-wide. qPCR for locus-specific.
5. Bioinformatics. Peak-calling pipelines specific to MeRIP-seq.

RayBiotech offers m6A antibodies. EpigenTek provides complete MeRIP kit workflows.