Methylated DNA Immunoprecipitation (MeDIP)

MeDIP kits for genome-wide DNA methylation profiling at lower sequencing cost

When you want genome-wide or locus-specific methylation profiling at lower cost than WGBS, MeDIP enriches methylcytosine-containing DNA fragments using anti-5mC antibodies. Retains genome-wide coverage with lower per-sample sequencing cost at the cost of single-base resolution.

Pick by mark and input.

1. Target mark. 5mC versus 5hmC requires different antibodies and protocols.
2. Input. Standard MeDIP needs micrograms. Low-input variants down to nanogram-scale.
3. Downstream. qPCR for locus-specific. Sequencing for genome-wide.
4. Caveat. Antibody bias toward dense methylation domains means absolute single-CpG quantification requires bisulfite-based methods.
5. Use case. Cost-effective genome-wide enrichment, not absolute CpG-level resolution.

EpigenTek leads MeDIP kits. RayBiotech offers complementary 5mC ELISA quantification.