Validated antibodies and ELISA kits for measuring histone phosphorylation marks
When you need to measure histone phosphorylation marks (H3S10ph mitosis, gamma-H2AX DNA damage, H3T3ph mitotic chromatin), this section covers antibodies, ELISA kits, and substrate reagents. Phospho-antibody specificity is notorious for cross-reactivity; validated antibodies matter.
Pick by mark and format.
- Mark. H3S10ph for mitosis. Gamma-H2AX for DNA double-strand breaks. H3T3ph for mitotic chromatin states.
- Format. Western blot, ELISA, immunofluorescence, flow cytometry each have a role.
- Antibody validation. Phospho-antibody specificity varies. Validated antibodies essential.
- DNA damage assays. Gamma-H2AX immunofluorescence remains gold standard for cleanest per-cell resolution. ELISA-based bulk quantification has lower resolution.
- Multiplex. For combined PTM panels match conjugate compatibility.
EpigenTek leads with phospho-histone ELISA kits and antibody panels.
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