Histone Acetylation

Histone acetylation assay kits, antibodies, and substrates for HAT and HDAC activity

When you need to measure or modulate acetylation of lysine residues on histones, this section covers it. Generally activating, acetylation marks enhancers and active promoters; H3K27ac specifically defines active enhancers. The category includes HAT and HDAC activity assay kits, acetylation-specific antibodies, peptide and nucleosome substrates, and global acetylation kits.

Pick by mark and enzyme class.

1. Mark. Different lysines have different functional readouts. H3K27ac for enhancer biology. H4K16ac for ageing and DNA damage.
2. Format. Peptide-based fluorogenic for kinetics. Nucleosome-based for physiological enzyme behaviour. ELISA for global. ChIP-grade for genome-wide.
3. Enzyme class. HATs versus the 18 HDACs across four classes. HDAC inhibitors show class selectivity.
4. Validation. Antibodies must distinguish closely related marks. Test specificity with peptide arrays.
5. Drug profiling. Profile against multiple HDAC isoforms; broad-spectrum versus selective inhibitors have different therapeutic profiles.

EpigenTek dominates global ELISA and activity-assay formats. Elabscience offers acetylated-histone ELISA and antibody pairs.