Exosome Depletion

Exosome-Depleted FBS and EV Depletion Kits for Clean Cell Culture Media

Removing background bovine vesicles from FBS in under two hours lets you run exosome secretion or uptake experiments against a clean baseline, without the 18-hour ultracentrifugation protocol that degrades growth factors. Affinity column and magnetic slurry depletion kits remove endogenous bovine exosomes from FBS, each processing up to 100 ml per run and validated by nanoparticle tracking analysis to show greater than 90% reduction in particles below 200 nm. Because residual particles remain, preparations should be described as EV-depleted rather than EV-free. For lot-to-lot consistency without depleting your own FBS, Exosome-depleted FBS Media Supplement (Exo-FBS from SBI System Biosciences) is available pre-validated for absence of bovine CD63 exosomes and bovine microRNAs.

Pick depletion method.

1. Ultracentrifugation. Cleanest depletion. Slow.
2. Polymer precipitation. Fastest.
3. Size-exclusion. Intermediate.
4. Verification. Verify depletion by Western for canonical exosome markers. Partial depletion contaminates downstream preps.
5. Cell growth. Verify depleted FBS still supports cell growth before scaling.

Norgen Biotek supplies exosome depletion reagents.