Enhanced Episomal Vectors (EEV)

Enhanced episomal vectors for sustained, integration-free transgene expression

When you want sustained transgene expression without genomic integration, EEV uses EBV oriP/EBNA-1 replication elements to maintain episomal persistence through cell divisions. Useful for footprint-free iPSC reprogramming and any application where integration is undesirable.

Pick by application.

1. iPSC reprogramming. EEV delivers footprint-free reprogramming without the lengthy excision step of PiggyBac.
2. Long-term transient expression. EEV maintains episomal presence through cell divisions.
3. Replication system. EBV oriP/EBNA-1 is dominant. Alternative systems for non-EBNA-1-compatible cells.
4. Selection marker. Puromycin or fluorescent reporter for selecting episome-positive cells.
5. Persistence duration. Months in dividing cells if selection is maintained.

SBI System Biosciences supplies EEV systems.