DNA methylation quantification kits for global and locus-specific 5mC and 5hmC analysis
When you need global or locus-specific 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) DNA methylation levels, this section covers ELISA-format global quantification, bisulfite-based locus-specific PCR or sequencing, methylated-DNA immunoprecipitation (MeDIP), and methylation-sensitive restriction digestion.
Pick by scope.
- Global %5mC. ELISA for fastest readout.
- Locus-specific quantitative. Bisulfite-PCR plus pyrosequencing.
- Genome-wide. Whole-genome bisulfite sequencing (WGBS) for full coverage. Reduced-representation BS-seq (RRBS) for cheaper CpG-focused.
- 5mC versus 5hmC. Mark-specific antibodies and protocols.
- Input. Microgram-scale for early methods. Nanogram-scale for newer kits.
EpigenTek dominates global ELISA and bisulfite-clean-up reagents.
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