DNA Methylation Detection

DNA methylation detection by methylation-specific PCR, restriction digestion, and pyrosequencing

When you need to detect specific DNA methylation patterns at defined loci for screening or diagnostic development, this section covers methylation-specific PCR, methylation-sensitive restriction digestion, and pyrosequencing-based methylation quantification.

Pick by method and resolution.

1. Method. Methylation-specific PCR fastest and cheapest but binary. Methylation-sensitive restriction-PCR gives proportional readout. Pyrosequencing gives single-base resolution at higher cost.
2. Diagnostic development. Methylation-specific PCR is the simplest first-pass screen. Confirm hits with pyrosequencing before clinical validation.
3. Multi-locus. Some platforms support parallel multi-locus detection.
4. Cost per sample. Match throughput to budget.

EpigenTek leads with EpiTect-style methylation detection kits.